The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.
Identifieur interne : 000501 ( Main/Exploration ); précédent : 000500; suivant : 000502The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.
Auteurs : Anna Moseler [Allemagne] ; Isabel Aller [Allemagne] ; Stephan Wagner [Allemagne] ; Thomas Nietzel [Allemagne] ; Jonathan Przybyla-Toscano [France] ; Ulrich Mühlenhoff [Allemagne] ; Roland Lill [Allemagne] ; Carsten Berndt [Allemagne] ; Nicolas Rouhier [Allemagne] ; Markus Schwarzl Nder [Allemagne] ; Andreas J. Meyer [Allemagne]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Arabidopsis.
- métabolisme : Arabidopsis, Ferrosulfoprotéines, Glutarédoxines, Mitochondries.
- Test de complémentation.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glutaredoxins, Iron-Sulfur Proteins.
- growth & development : Arabidopsis.
- metabolism : Arabidopsis, Mitochondria.
- Genetic Complementation Test.
Abstract
The iron-sulfur cluster (ISC) is an ancient and essential cofactor of many proteins involved in electron transfer and metabolic reactions. In Arabidopsis, three pathways exist for the maturation of iron-sulfur proteins in the cytosol, plastids, and mitochondria. We functionally characterized the role of mitochondrial glutaredoxin S15 (GRXS15) in biogenesis of ISC containing aconitase through a combination of genetic, physiological, and biochemical approaches. Two Arabidopsis T-DNA insertion mutants were identified as null mutants with early embryonic lethal phenotypes that could be rescued by GRXS15. Furthermore, we showed that recombinant GRXS15 is able to coordinate and transfer an ISC and that this coordination depends on reduced glutathione (GSH). We found the Arabidopsis GRXS15 able to complement growth defects based on disturbed ISC protein assembly of a yeast Δgrx5 mutant. Modeling of GRXS15 onto the crystal structures of related nonplant proteins highlighted amino acid residues that after mutation diminished GSH and subsequently ISC coordination, as well as the ability to rescue the yeast mutant. When used for plant complementation, one of these mutant variants, GRXS15K83/A, led to severe developmental delay and a pronounced decrease in aconitase activity by approximately 65%. These results indicate that mitochondrial GRXS15 is an essential protein in Arabidopsis, required for full activity of iron-sulfur proteins.
DOI: 10.1073/pnas.1510835112
PubMed: 26483494
PubMed Central: PMC4640787
Affiliations:
- Allemagne, France
- District de Cologne, District de Düsseldorf, District de Giessen, Grand Est, Hesse (Land), Lorraine (région), Rhénanie-du-Nord-Westphalie
- Bonn, Champenoux, Düsseldorf, Juliers, Marbourg
- Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.</title><author><name sortKey="Moseler, Anna" sort="Moseler, Anna" uniqKey="Moseler A" first="Anna" last="Moseler">Anna Moseler</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Aller, Isabel" sort="Aller, Isabel" uniqKey="Aller I" first="Isabel" last="Aller">Isabel Aller</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Wagner, Stephan" sort="Wagner, Stephan" uniqKey="Wagner S" first="Stephan" last="Wagner">Stephan Wagner</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Nietzel, Thomas" sort="Nietzel, Thomas" uniqKey="Nietzel T" first="Thomas" last="Nietzel">Thomas Nietzel</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Przybyla Toscano, Jonathan" sort="Przybyla Toscano, Jonathan" uniqKey="Przybyla Toscano J" first="Jonathan" last="Przybyla-Toscano">Jonathan Przybyla-Toscano</name><affiliation wicri:level="4"><nlm:affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Muhlenhoff, Ulrich" sort="Muhlenhoff, Ulrich" uniqKey="Muhlenhoff U" first="Ulrich" last="Mühlenhoff">Ulrich Mühlenhoff</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Lill, Roland" sort="Lill, Roland" uniqKey="Lill R" first="Roland" last="Lill">Roland Lill</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Berndt, Carsten" sort="Berndt, Carsten" uniqKey="Berndt C" first="Carsten" last="Berndt">Carsten Berndt</name><affiliation wicri:level="3"><nlm:affiliation>Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation wicri:level="4"><nlm:affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France; Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France; Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Schwarzl Nder, Markus" sort="Schwarzl Nder, Markus" uniqKey="Schwarzl Nder M" first="Markus" last="Schwarzl Nder">Markus Schwarzl Nder</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany; Bioeconomy Science Center, c/o Forschungszentrum Jülich, 52425 Jülich, Germany andreas.meyer@uni-bonn.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany; Bioeconomy Science Center, c/o Forschungszentrum Jülich, 52425 Jülich</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Juliers</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26483494</idno><idno type="pmid">26483494</idno><idno type="doi">10.1073/pnas.1510835112</idno><idno type="pmc">PMC4640787</idno><idno type="wicri:Area/Main/Corpus">000495</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000495</idno><idno type="wicri:Area/Main/Curation">000495</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000495</idno><idno type="wicri:Area/Main/Exploration">000495</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.</title><author><name sortKey="Moseler, Anna" sort="Moseler, Anna" uniqKey="Moseler A" first="Anna" last="Moseler">Anna Moseler</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Aller, Isabel" sort="Aller, Isabel" uniqKey="Aller I" first="Isabel" last="Aller">Isabel Aller</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Wagner, Stephan" sort="Wagner, Stephan" uniqKey="Wagner S" first="Stephan" last="Wagner">Stephan Wagner</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Nietzel, Thomas" sort="Nietzel, Thomas" uniqKey="Nietzel T" first="Thomas" last="Nietzel">Thomas Nietzel</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Przybyla Toscano, Jonathan" sort="Przybyla Toscano, Jonathan" uniqKey="Przybyla Toscano J" first="Jonathan" last="Przybyla-Toscano">Jonathan Przybyla-Toscano</name><affiliation wicri:level="4"><nlm:affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Champenoux</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Muhlenhoff, Ulrich" sort="Muhlenhoff, Ulrich" uniqKey="Muhlenhoff U" first="Ulrich" last="Mühlenhoff">Ulrich Mühlenhoff</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Lill, Roland" sort="Lill, Roland" uniqKey="Lill R" first="Roland" last="Lill">Roland Lill</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Berndt, Carsten" sort="Berndt, Carsten" uniqKey="Berndt C" first="Carsten" last="Berndt">Carsten Berndt</name><affiliation wicri:level="3"><nlm:affiliation>Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation wicri:level="4"><nlm:affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France; Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France; Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Schwarzl Nder, Markus" sort="Schwarzl Nder, Markus" uniqKey="Schwarzl Nder M" first="Markus" last="Schwarzl Nder">Markus Schwarzl Nder</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany; Bioeconomy Science Center, c/o Forschungszentrum Jülich, 52425 Jülich, Germany andreas.meyer@uni-bonn.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany; Bioeconomy Science Center, c/o Forschungszentrum Jülich, 52425 Jülich</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Juliers</settlement></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (growth & development)</term><term>Arabidopsis (metabolism)</term><term>Genetic Complementation Test (MeSH)</term><term>Glutaredoxins (metabolism)</term><term>Iron-Sulfur Proteins (metabolism)</term><term>Mitochondria (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (métabolisme)</term><term>Ferrosulfoprotéines (métabolisme)</term><term>Glutarédoxines (métabolisme)</term><term>Mitochondries (métabolisme)</term><term>Test de complémentation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutaredoxins</term><term>Iron-Sulfur Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Ferrosulfoprotéines</term><term>Glutarédoxines</term><term>Mitochondries</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Complementation Test</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Test de complémentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The iron-sulfur cluster (ISC) is an ancient and essential cofactor of many proteins involved in electron transfer and metabolic reactions. In Arabidopsis, three pathways exist for the maturation of iron-sulfur proteins in the cytosol, plastids, and mitochondria. We functionally characterized the role of mitochondrial glutaredoxin S15 (GRXS15) in biogenesis of ISC containing aconitase through a combination of genetic, physiological, and biochemical approaches. Two Arabidopsis T-DNA insertion mutants were identified as null mutants with early embryonic lethal phenotypes that could be rescued by GRXS15. Furthermore, we showed that recombinant GRXS15 is able to coordinate and transfer an ISC and that this coordination depends on reduced glutathione (GSH). We found the Arabidopsis GRXS15 able to complement growth defects based on disturbed ISC protein assembly of a yeast Δgrx5 mutant. Modeling of GRXS15 onto the crystal structures of related nonplant proteins highlighted amino acid residues that after mutation diminished GSH and subsequently ISC coordination, as well as the ability to rescue the yeast mutant. When used for plant complementation, one of these mutant variants, GRXS15K83/A, led to severe developmental delay and a pronounced decrease in aconitase activity by approximately 65%. These results indicate that mitochondrial GRXS15 is an essential protein in Arabidopsis, required for full activity of iron-sulfur proteins. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26483494</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>112</Volume><Issue>44</Issue><PubDate><Year>2015</Year><Month>Nov</Month><Day>03</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.</ArticleTitle><Pagination><MedlinePgn>13735-40</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1510835112</ELocationID><Abstract><AbstractText>The iron-sulfur cluster (ISC) is an ancient and essential cofactor of many proteins involved in electron transfer and metabolic reactions. In Arabidopsis, three pathways exist for the maturation of iron-sulfur proteins in the cytosol, plastids, and mitochondria. We functionally characterized the role of mitochondrial glutaredoxin S15 (GRXS15) in biogenesis of ISC containing aconitase through a combination of genetic, physiological, and biochemical approaches. Two Arabidopsis T-DNA insertion mutants were identified as null mutants with early embryonic lethal phenotypes that could be rescued by GRXS15. Furthermore, we showed that recombinant GRXS15 is able to coordinate and transfer an ISC and that this coordination depends on reduced glutathione (GSH). We found the Arabidopsis GRXS15 able to complement growth defects based on disturbed ISC protein assembly of a yeast Δgrx5 mutant. Modeling of GRXS15 onto the crystal structures of related nonplant proteins highlighted amino acid residues that after mutation diminished GSH and subsequently ISC coordination, as well as the ability to rescue the yeast mutant. When used for plant complementation, one of these mutant variants, GRXS15K83/A, led to severe developmental delay and a pronounced decrease in aconitase activity by approximately 65%. These results indicate that mitochondrial GRXS15 is an essential protein in Arabidopsis, required for full activity of iron-sulfur proteins. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Moseler</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aller</LastName><ForeName>Isabel</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wagner</LastName><ForeName>Stephan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nietzel</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Przybyla-Toscano</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mühlenhoff</LastName><ForeName>Ulrich</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lill</LastName><ForeName>Roland</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berndt</LastName><ForeName>Carsten</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Neurology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouhier</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Unité Mixte de Recherche (UMR) 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France; UMR 1136 Interactions Arbres/Microorganismes, Centre Institut National de la Recherche Agronomique (INRA) Nancy Lorraine, INRA, 54280 Champenoux, France; Institut für Zytobiologie, Philipps-Universität Marburg, 35032 Marburg, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schwarzländer</LastName><ForeName>Markus</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>INRES-Plant Energy Biology, University of Bonn, 53113 Bonn, Germany;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>Andreas J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation (INRES)-Chemical Signalling, University of Bonn, 53113 Bonn, Germany; Bioeconomy Science Center, c/o Forschungszentrum Jülich, 52425 Jülich, Germany andreas.meyer@uni-bonn.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007506">Iron-Sulfur Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005816" MajorTopicYN="N">Genetic Complementation Test</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007506" MajorTopicYN="N">Iron-Sulfur Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">aconitase</Keyword><Keyword MajorTopicYN="N">glutaredoxin</Keyword><Keyword MajorTopicYN="N">glutathione</Keyword><Keyword MajorTopicYN="N">iron-sulfur cluster</Keyword><Keyword MajorTopicYN="N">mitochondria</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26483494</ArticleId><ArticleId IdType="pii">1510835112</ArticleId><ArticleId IdType="doi">10.1073/pnas.1510835112</ArticleId><ArticleId IdType="pmc">PMC4640787</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 2014 Feb;164(2):637-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24351685</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Mar;45(5):775-88</Citation><ArticleIdList><ArticleId IdType="pubmed">16460511</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2013 Oct 9;135(40):15153-64</Citation><ArticleIdList><ArticleId IdType="pubmed">24032439</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Jul 29;333(6042):601-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21798944</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20532-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22139372</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Jul 1;280(26):24544-52</Citation><ArticleIdList><ArticleId IdType="pubmed">15833738</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2008;59(14):4017-27</Citation><ArticleIdList><ArticleId IdType="pubmed">18977750</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2014 Jan;7(1):187-205</Citation><ArticleIdList><ArticleId IdType="pubmed">24203231</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2014 Aug 29;289(35):24588-98</Citation><ArticleIdList><ArticleId IdType="pubmed">25012657</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1998 Dec;16(6):735-43</Citation><ArticleIdList><ArticleId IdType="pubmed">10069079</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2008;59:143-66</Citation><ArticleIdList><ArticleId IdType="pubmed">18444899</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Dec 16;4:506</Citation><ArticleIdList><ArticleId IdType="pubmed">24379821</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Apr;167(4):1643-58</Citation><ArticleIdList><ArticleId IdType="pubmed">25699589</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2009 Feb;57(3):534-41</Citation><ArticleIdList><ArticleId IdType="pubmed">18939964</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2005 Aug 18;436(7053):1035-39</Citation><ArticleIdList><ArticleId IdType="pubmed">16110529</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2642-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14983062</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2008 Apr 9;27(7):1122-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18354500</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2003 Jul 11;278(28):25745-51</Citation><ArticleIdList><ArticleId IdType="pubmed">12730244</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2009 May;1787(5):468-75</Citation><ArticleIdList><ArticleId IdType="pubmed">19366606</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2007 Jan;9(1):151-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17115894</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2007;2(1):31-4</Citation><ArticleIdList><ArticleId IdType="pubmed">17401334</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2002 Oct 4;277(40):37590-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12138088</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2008 Mar 19;582(6):848-54</Citation><ArticleIdList><ArticleId IdType="pubmed">18275854</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2014 Sep 23;53(37):5834-47</Citation><ArticleIdList><ArticleId IdType="pubmed">25153801</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1999 Oct;20(2):251-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10571885</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2002 May;7(5):193-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11992820</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2003 Oct;133(2):462-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14555774</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2014 Aug 22;289(34):23264-74</Citation><ArticleIdList><ArticleId IdType="pubmed">25006243</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2002 Apr;13(4):1109-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11950925</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Jul 24;4:259</Citation><ArticleIdList><ArticleId IdType="pubmed">23898337</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Oct;157(2):587-98</Citation><ArticleIdList><ArticleId IdType="pubmed">21841088</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2007 Dec 25;46(51):15018-26</Citation><ArticleIdList><ArticleId IdType="pubmed">18044966</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2003 Nov 21;278(47):46869-77</Citation><ArticleIdList><ArticleId IdType="pubmed">12954611</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2006 Feb 22;25(4):900-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16437155</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 Feb;152(2):736-46</Citation><ArticleIdList><ArticleId IdType="pubmed">20007440</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2007;372:325-42</Citation><ArticleIdList><ArticleId IdType="pubmed">18314737</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2012 Jan;63(1):403-11</Citation><ArticleIdList><ArticleId IdType="pubmed">21984653</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2006 Dec;48(6):873-82</Citation><ArticleIdList><ArticleId IdType="pubmed">17092311</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 Dec;52(5):973-86</Citation><ArticleIdList><ArticleId IdType="pubmed">17892447</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2009 Aug;66(15):2539-57</Citation><ArticleIdList><ArticleId IdType="pubmed">19506802</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 1999 Dec;19(12):8180-90</Citation><ArticleIdList><ArticleId IdType="pubmed">10567543</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 Mar;152(3):1514-28</Citation><ArticleIdList><ArticleId IdType="pubmed">20089767</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2013 Aug;162(4):1822-33</Citation><ArticleIdList><ArticleId IdType="pubmed">23660836</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Hum Genet. 2011 Oct 7;89(4):486-95</Citation><ArticleIdList><ArticleId IdType="pubmed">21944046</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1991 Mar 25;19(6):1349</Citation><ArticleIdList><ArticleId IdType="pubmed">2030957</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2013 Jan 4;12(1):491-504</Citation><ArticleIdList><ArticleId IdType="pubmed">23198870</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>France</li></country><region><li>District de Cologne</li><li>District de Düsseldorf</li><li>District de Giessen</li><li>Grand Est</li><li>Hesse (Land)</li><li>Lorraine (région)</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Bonn</li><li>Champenoux</li><li>Düsseldorf</li><li>Juliers</li><li>Marbourg</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Moseler, Anna" sort="Moseler, Anna" uniqKey="Moseler A" first="Anna" last="Moseler">Anna Moseler</name></region><name sortKey="Aller, Isabel" sort="Aller, Isabel" uniqKey="Aller I" first="Isabel" last="Aller">Isabel Aller</name><name sortKey="Berndt, Carsten" sort="Berndt, Carsten" uniqKey="Berndt C" first="Carsten" last="Berndt">Carsten Berndt</name><name sortKey="Lill, Roland" sort="Lill, Roland" uniqKey="Lill R" first="Roland" last="Lill">Roland Lill</name><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name><name sortKey="Muhlenhoff, Ulrich" sort="Muhlenhoff, Ulrich" uniqKey="Muhlenhoff U" first="Ulrich" last="Mühlenhoff">Ulrich Mühlenhoff</name><name sortKey="Nietzel, Thomas" sort="Nietzel, Thomas" uniqKey="Nietzel T" first="Thomas" last="Nietzel">Thomas Nietzel</name><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><name sortKey="Schwarzl Nder, Markus" sort="Schwarzl Nder, Markus" uniqKey="Schwarzl Nder M" first="Markus" last="Schwarzl Nder">Markus Schwarzl Nder</name><name sortKey="Wagner, Stephan" sort="Wagner, Stephan" uniqKey="Wagner S" first="Stephan" last="Wagner">Stephan Wagner</name></country><country name="France"><region name="Grand Est"><name sortKey="Przybyla Toscano, Jonathan" sort="Przybyla Toscano, Jonathan" uniqKey="Przybyla Toscano J" first="Jonathan" last="Przybyla-Toscano">Jonathan Przybyla-Toscano</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000501 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000501 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GlutaredoxinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26483494
|texte= The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26483494" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GlutaredoxinV1
| This area was generated with Dilib version V0.6.37. |  |